Comparison of Bromcresol Purple and Capillary Protein Electrophoresis for Quantification of Serum Albumin in Multiple Myeloma

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Comparison of bromcresol purple and capillary protein electrophoresis for quantification of serum albumin in multiple myeloma.

Employment or Leadership: None declared. Consultant or Advisory Role: None declared. Stock Ownership: In accordance with the Bayh–Dole Act of 1980 and Mayo Foundation policy, the authors disclose that V.A. Lennon, as inventor, stands to receive royalties for commercial assays to detect aquaporin-4– specific autoantibodies. The intellectual property is licensed to a commercial entity for the dev...

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Comparison of bromcresol green and agarose protein electrophoresis for quantitation of serum albumin in multiple myeloma.

BACKGROUND The International Staging System for multiple myeloma has increased the importance of accurate measurement of serum albumin. Two common albumin assays, bromcresol green (BCG) and agarose gel protein electrophoresis (PEL), frequently yield discordant results, creating confusion regarding which assay is superior for use in myeloma. METHODS We measured albumin by BCG on a Roche Modula...

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Measurement of serum albumin by capillary zone electrophoresis, bromocresol green, bromocresol purple, and immunoassay methods.

BACKGROUND/AIMS The introduction of capillary zone electrophoresis (CZE) to this laboratory has highlighted discrepancies in albumin measured on an Abbott Aeroset by bromocresol green (BCG) and that calculated by CZE on the basis of total protein measured by Biuret. METHODS This study examined differences in albumin estimation by CZE, Abbott Aeroset BCG, and Aeroset bromocresol purple (BCP), ...

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New automated dye-binding method for serum albumin determination with bromcresol purple.

We describe a new automated dye-binding method for serum albumin determination with bromcresol purple (BCP) that has several advantages over an existing bromcresol green (BCG) method. The continuous-flow method is sensitive, linear, and precise, with negligible sample interaction at an analytical rate of 60 samples per hour. Unlike BCG, BCP did not react with an albumin-free serum globulin prep...

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ژورنال

عنوان ژورنال: Clinical Chemistry

سال: 2009

ISSN: 0009-9147,1530-8561

DOI: 10.1373/clinchem.2008.117119